The theory of evolution does not permit the existence of any counterexamples. If any one of the 48 counterexamples listed below is correct, then the theory of evolution fails. Moreover, even if there is merely a 5% chance that each of these counterexamples is correct (and the odds are far higher than that), then the probability that the theory of evolution is true is less than 9%.
- The current annual rate of extinction of species far exceeds any plausible rate of generation of species. Expanding the amount of time for evolution to occur makes evolution even less likely.
- Evolution cannot explain artistic beauty, such as the brilliant autumn foliage and staggering array of beautiful marine fish, both of which originated before any human to view them; this lacks any plausible evolutionary explanation apart from sexual selection. Although the idea of artistic beauty is a purely human concept developed from natural objects.
- Evolution cannot explain the lack of genetic diversity among the Homo sapiens species. Were evolution and the Old Earth theory true, the human population would show a much larger genetic variance. Some scientists have stated that a troop of 55 chimpanzees contains more genetic diversity than the entire human race; this would support the idea that all chimps are descended from a relatively large initial population while all humans are descended from a much smaller initial population (two people, perhaps). 80% of all human diversity is found on the African continent, which accords with a human population growing from a small group in the post-Flood Middle East.
feathers are a problem for evolutionists. For more information, please see parrot featherssee picture source and license here
- Parsimonious repetition of design elements throughout Creation, e.g. the eye's appearance in remarkably different species. For such complex structures to arise repeatedly via evolution is impossible, as evolution is an inherently random and historically contingent process.
- Pleiotropy, the fact that a change of a single gene can have several different effects, renders the "improvement" of animals by random mutation impossible, as any mutation with a potentially beneficial effect will be coupled with one or more other potentially lethal effects.
- The development of feathers, which could not have conceivably "grown" from the scales of reptiles
- For evolution to be true, every male dog, cat, horse, elephant, giraffe, fish and bird had to have coincidentally evolved with a female alongside it (over billions of years) with fully evolved compatible reproductive parts and a desire to mate, otherwise the species couldn't keep going. Although the changes that occur after one generation are definitely not great enough to stop to organisms from reproducing. 
- There are no historical records of anyone directly observing one species evolving into another, which would certainly be something worth writing about. Surely of the millions of species we have, someone would have witnessed one come into existence had it evolved. Although the time humans have been keeping is not nearly great enough to show any significant change. And evolution is a relatively slow process which does not spontaneously occur. 
- If evolution were to explain where human beings come from, then every personality type should benefit human life.  This is clearly untrue because the world is filled with liars, psychopaths, murderers and creationists. These traits clearly do not benefit humanity. This point is to do with social evolution where a large society cannot exist where these traits are accepted. This is generally accepted as the source of the so called morals.
- Evolution should lead to the extinction of homosexuality, as sinful homosexuals are unfit to contribute to the gene pool, yet to this day homosexuality continues to exist. This does idea does however need homosexuality to be heritable.
- The Theory of Evolution dictates that all organisms descended from single celled bacteria. Considering that bacteria is, and always has been, the most successful group of organisms, covering all areas of the globe in some of the most extreme environments, why would it have been advantageous to evolve into organisms that are so much more limited to the environments they can inhabit? Surely, staying as bacteria would be far more advantageous, due to their tolerence(sp) of extreme conditions, quick reproduction time, etc. Evolution does not state that life all evolved from one bacteria. And bacteria rarely occupy the same ecological niche as other more complex life forms.
- The Second Law of Thermodynamics establishes that everything becomes more disordered over time, in a closed system; the theory of evolution claims that some systems can become more ordered. This actually does not subvert the Second Law of Thermodynamics. By that logic the building industry could not exist as a building is more ordered than the clay in ground. And if sand falls out of my hand onto the ground it forms a cone which is more ordered than the handful of sand. Although the second law of thermodynamics refers to the overall disorder of the energy in the universe.
- The Law of Large Numbers states that things tend to revert to their average over time (the large number of examples), while evolution requires the opposite: that things become more complex and depart further from their average over time and large numbers of examples.
Lack of mechanism
biologist Ernst Mayr
wrote: "It must be admitted, however, that it is a considerable strain on one’s credulity to assume that finely balanced systems such as certain sense organs (the eye of vertebrates, or the bird’s feather) could be improved by random mutations
- The extraordinary migration patterns of butterflies and birds cannot be explained through naturalistic development, and lack any plausible materialistic explanation
- Evolution does not account for the immense amount of information in the genome. While there are various definitions of information, and many types have been observed to occur naturally, DNA contains information that is processed to lead to a result predetermined by the content of that information. Strictly speaking it is inaccurate to refer to DNA as a "code" or "language," as many scientists are prone to doing. In fact DNA is more like a template, which produces messenger RNA (mRNA,) a new template with more appropriate bases for protein production. The mRNA essentially acts as a scaffold to which the appropriate amino acids attach to form a protein molecule. Rather than being a language containing words which each have a meaning, DNA is more like a jig or framework which allows a specific molecule of mRNA, and subsequently a specific protein sequence, to be assembled on it. In effect the information is the sequence of chemical reactions which that length of DNA will catalyse. Given the huge number of useless protein molecules which could be formed and the complexity of even a simple protein such as haemoglobin, this sequence could not have evolved by natural selection as the odds against the initial organism having a functional protein are too great.
- The development of feathers, which could not have conceivably "grown" from the scales of reptiles or any other known structure.
- Humans exhibit behaviors such as performing science, creating art and music, dancing, and a number of other intellectual and artistic behaviors which could not have been produced by random mutations. There is no known evolutionary reason why these should be favored.
- Trematode parasites, like many other kinds, lack a plausible evolutionary phylogeny, though they can easily be explained by a teleological design.
- Evolution cannot explain the many complex sex-determining systems. For example, in most mammals, females have two identical sex chromosomes (XX in this case) whereas males two different ones (XY.) However in birds, reptiles, many insects, and other organisms, the situation is reversed to where the male has two identical sex chromosomes and the female has two different ones; for example male birds have a ZZ chromosome pair and females ZW. No evolutionist has proposed a mechanism by which mammals could have a different sex chromosome system from the reptile ancestors they allegedly share with birds.
- Evolution requires that random mutations can cause one kind to change into another, but this has never been observed.
- The existence of two symmetrical kidneys, which are unnecessary in most people, lacks a plausible evolutionary explanation based on functionality alone. Because evolution falsehoods mislead most people into thinking they need their second kidney, "the average waiting time for the organs from a deceased donor in the United States is five years" and "3,916 patients waiting for a kidney in 2006 died before one became available."
- Thousands of years of intense selective breeding should have produced a new wolf species from domesticated dogs, yet dogs and wolves remain the same species.
- Inability to account for widely observed altruism among animals, as it reduces an animal's ability to survive. “The existence of altruism between different species — which is not uncommon — remains an obstinate enigma.” Not surprisingly, many atheist evolutionists have done their utmost to deny animal altruism.
- The gradual buildup of deleterious mutations in an organism's genome as the species grows older limits the maximum lifespan of a species well under what would be required for evolution. Each generation of humans has far more negative mutations than the posited natural selection can remove. Evolution is thus impossible as species would become nonviable long before they could diverge.
- Lack of any demonstrable vestigial parts of the human genome. While evolutionists often claim that regions of the genome are "junk DNA" and would not have been placed there by a designer, none have actually shown this to be true, and much so-called "junk DNA" has been shown to be useful.
- While evolutionists argue that there are examples of "bad design" in the bodies of many organisms, such as "flaws" in the human spine and sinus system, evolutionists fail to realize that, by their own theory, natural selection should have removed these things! The simpler explanation, that these represent degeneration from an original, created perfect form, is the superior one. In other word, as CreationWiki notes, such "flaws" are actually "a result of deterioration, resulting from Man's Fall."
- We have the perfect number of teeth to fit in our mouths. While creationism perfectly accounts for that result, evolutionism predicts a contrary result: As our faces evolved from chimpanzee-like faces to human faces, the shortening of the muzzle would have caused the teeth to become badly overcrowded in the front of the mouth.
- Human fertility is rapidly declining, disproving evolutionary improvement in humans and also suggesting a brief timeline for human existence.
- Evolutionist theory predicts that in the case of convergent evolution, a particular structure such as an eye that evolves in an optimal form in one species can later evolve in a suboptimal form in a different species. No such result has ever been observed.
- Constantly mutating, drug-resistant pathogens such as MRSA have been demonstrated to be the result of devolution rather than evolution. This is the exact opposite of what evolutionary theory predicts.
The remarkable whale
, which is a mammal
, has no plausible evolutionary ancestor.
- No transitional forms appear for horses, instead different and distinct horse-like animals appear in the fossil record
- The enormous gaps and lack of intermediate species in the fossil record, once all the frauds are removed.
- Mammalian fur and body hair. There is no known evolutionary pathway for the development of fur, and no fossil evidence of hair evolving from scales, even though it survives very well.
- The extraordinarily long neck of the giraffe. The giraffe's heart creates immense pressure to drive blood up the neck to the brain. Because of this there are valves in the neck which automatically restrict the blood flow when the giraffe lowers its head to drink. Without these valves the sudden increase in blood pressure as the heart no longer needs to overcome gravity would rupture the arteries in the brain and kill the giraffe. However the giraffe could not have evolved a long neck without the valves and had no need to evolve the valves unless it had a long neck. The okapi, which evolutionists claim is the closest relative of the giraffe, has no such valves. Evolutionary theory cannot explain this. 
- The development of wings in birds, as intermediary wing stubs would have no use, and be a competitive disadvantage.
- The flagellum of certain bacteria contain a multi-part cellular motor which fails to function if a single part is removed. This is the classic example of irreducible complexity as publicised by Professor Michael Behe. Because the flagellum must have all its parts to function it could not have evolved and therefore must have been designed by an intelligent being. At the Kitzmiller vs. Dover Area School District trial, Professor Ken Miller attempted to rebut this argument by pointing out that if 42 parts of the flagellum are removed what remains is a fully functional Type III Secretory System, used by some bacteria to inject toxins into target cells. While Miller's claim is factually accurate, it fails to explain how the T3SS could simultaneously add 42 parts to create a working flagellum.
- The organ and brain development required for retinal imagery require a base level of complexity, making a primitive form useless and impossible under evolution.
- The ear contains three tiny bones that transmit sound waves from the eardrum to the cochlea. Because of the complicated arrangement of those bones, transitional forms (which have never been found) would have served no purpose. Evolutionists claim that these bones used to form part of the reptilian jaw joint, but no intermediate fossil with half jaw/half ear has ever been found, and there is no way evolutionism can explain how a jaw becomes an ear.
- Bony skeletons represent an example of irreducible complexity, since only a fully formed, complete skeleton is of any use whatsoever, while having only one or some few number of bones in an otherwise invertebrate creature is hardly advantageous. It is inconceivable to think that some random mutation could have resulted in the formation if an entire bone system in a creature which was previously invertebrate.
- "Social insects" such as bees, ants, and termites, which have extremely complex caste systems. Where an insect fits into the system is determined by its diet when young. Evolution requires that the genes for the various castes and the genes for caste-specific behavior all appeared simultaneously. Akin to the concept of irreducible complexity, but on a higher (social) order, this is impossible given random chance. The complex social structure of these insects is evidence of intelligent design; also, since the overwhelming majority of individuals are workers and do not reproduce, they do not perpetuate their genetic material, as evolutionism claims all living things must do.
- Lungs never would have had to develop unless the animal was already on land, in which case, they would die before reproducing.
- There is no explanation within the theory of evolution through natural selection for the mechanism in which a catapillar turns into a butterfly. Essentially the original animal is broken down and rearranged into a totally different animal, with no simmilar physical characteristics. This process cannot be explained within the theory, and points to a designer's involvement.
- Evolution would result in modern languages having one common ancestral language, and for nearly a century linguists insisted that there must be one. There is not, and linguists now accept that there are completely independent families of languages.
- No other animal exhibits religion. A far better explanation than random mutations is that humans were given the capacity to be religious by a loving God who wants a relationship with His creations.
- Circadian phenomena -- internal 24-hour clock mechanisms of humans and other living beings -- defy material explanation. Examples include how some people are unable to change the timing of their need for sleep for each day, and how plants exhibit clock-like behavior regardless of their exposure to sunlight. In addition, there is a weekly clock cycle for many phenomena, which has a clear biblical basis but defies any materialistic explanation.
- Scientists have found proof that the first chicken came before the first egg, consistent with a special creation of chickens but not with a gradual descent with modifications from a proto-chicken and proto-egg.
- Despite the large (and ever increasing) number of breeds of dogs, and the fact that some pure bred lineages of dogs are reproductively isolated from other dogs, no new species of dog has evolved.
Question evolution! campaign
See also: Question evolution! campaign and Atheists doubting the validity of atheism
Since World War II a majority of the most prominent and vocal defenders of the theory of evolution which employs methodological naturalism have been atheists. In 2011, a campaign called the Question evolution! campaign was launched by the biblical creation organization Creation Ministries International which is a worldwide "grass-roots movement to challenge the anti-Christian dogma of evolution". The focus of the Question evolution! campaign is on "15 questions that evolutionists cannot satisfactorily answer." The 15 questions posed to evolutionists (and undecided individuals) can be found HERE The campaign will be primarily conducted in the United States, the United Kingdom, Canada, Europe, Australia, New Zealand, Singapore and South Africa.
As scientific theories require that their laws be immutable, the existence of merely one counterexample disproves the truth of the rule. Thus, if evolution fails to account for any one of these items (or countless others), it must be discarded.