Difference between revisions of "Polymerase chain reaction"
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'''Polymerase chain reaction'' or PCR refers to a method of heat cycling to amplify [[DNA]]. It was made possible by the discovery of a thermophilic (heat loving) bacterium: ''thermus aquaticus''. The DNA polymerase from this bacterium made DNA replication at high temperatures (60-70 degrees Celsius) possible. PCR consists of a melting period at 95 degrees Celsius, an annealing period at 55 degrees Celsius and a elongation period, at 68 degrees Celsius. | '''Polymerase chain reaction'' or PCR refers to a method of heat cycling to amplify [[DNA]]. It was made possible by the discovery of a thermophilic (heat loving) bacterium: ''thermus aquaticus''. The DNA polymerase from this bacterium made DNA replication at high temperatures (60-70 degrees Celsius) possible. PCR consists of a melting period at 95 degrees Celsius, an annealing period at 55 degrees Celsius and a elongation period, at 68 degrees Celsius. | ||
| − | PCR was first developed in 1983 by Kary Mullis.< | + | PCR was first developed in 1983 by Kary Mullis.<ref>Bartlett & Stirling (2003)—A Short History of the Polymerase Chain Reaction. In: Methods Mol Biol. 226:3-6</ref> |
| − | == | + | ==References== |
| − | + | <references /> | |
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Revision as of 22:40, June 23, 2008
'Polymerase chain reaction or PCR refers to a method of heat cycling to amplify DNA. It was made possible by the discovery of a thermophilic (heat loving) bacterium: thermus aquaticus. The DNA polymerase from this bacterium made DNA replication at high temperatures (60-70 degrees Celsius) possible. PCR consists of a melting period at 95 degrees Celsius, an annealing period at 55 degrees Celsius and a elongation period, at 68 degrees Celsius.
PCR was first developed in 1983 by Kary Mullis.[1]
References
- ↑ Bartlett & Stirling (2003)—A Short History of the Polymerase Chain Reaction. In: Methods Mol Biol. 226:3-6