Difference between revisions of "Transient expression"
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A '''transient expression''' is the short-term detectable expression of the product of a [[gene]] that has been transferred into protoplasts, cells, or plants. | A '''transient expression''' is the short-term detectable expression of the product of a [[gene]] that has been transferred into protoplasts, cells, or plants. | ||
| − | Marker genes on the [[DNA]] construct (such as | + | Marker genes on the [[DNA]] construct (such as -glucuronidase) that are readily assayed in plant tissues can be used to determine whether DNA has been transferred into the cells. |
Transient expression indicates only that the marker gene is transcribed and translated in the cells assayed. It does not imply that the gene or DNA construct has been heritably incorporated into the plant genome. | Transient expression indicates only that the marker gene is transcribed and translated in the cells assayed. It does not imply that the gene or DNA construct has been heritably incorporated into the plant genome. | ||
[[Category:Molecular Biology]] | [[Category:Molecular Biology]] | ||
Latest revision as of 03:26, September 11, 2016
A transient expression is the short-term detectable expression of the product of a gene that has been transferred into protoplasts, cells, or plants.
Marker genes on the DNA construct (such as -glucuronidase) that are readily assayed in plant tissues can be used to determine whether DNA has been transferred into the cells.
Transient expression indicates only that the marker gene is transcribed and translated in the cells assayed. It does not imply that the gene or DNA construct has been heritably incorporated into the plant genome.