Difference between revisions of "Chromosomes"

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Chromosome

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-{{merging|Chromosome}}
+#REDIRECT [[Chromosome]]
-'''Chromosomes''' (literally: ''colored bodies'', from [[Greek]]) are packaged units of [[DNA]] and associated proteins.<ref>http://learn.genetics.utah.edu/units/disorders/karyotype/whatarechrom.cfm</ref> The chromosome carries portions of the hereditary information of an organism.  The DNA is tightly coiled many times around proteins called [[histone]]s that support the chromosome structure.<ref>http://ghr.nlm.nih.gov/handbook/basics/chromosome</ref>  The bound DNA units are called [[nucleosome]]s.
-==Physical Characteristics of Chromosomes==
-Most chromosomes have the rough shape of an I when they condense prior to replication and are as bits of long invisible string when unwound. The familiar X shape actually refers to 2 identical chromosomes referred to as sister chromatids. Each chromosome has a constriction point called the [[centromere]], which divides the chromosome into two sections, or “arms.” The short arm of the chromosome is labeled the “p arm” for "petite". The long arm of the chromosome is labeled the “q arm” for "not petite". The location of the centromere on each chromosome gives the chromosome its characteristic shape, and can be used to help describe the location of specific genes.<ref>http://www.rothamsted.ac.uk/notebook/courses/guide/chromo.htm</ref>
-The chromosomal DNA molecule contains three specific [[nucleotide]] sequences which are required for replication: a DNA replication origin; the [[centromere]] to attach the DNA to the mitotic spindle.; a [[telomere]] located at each end of the linear chromosome. <ref>http://ghr.nlm.nih.gov/ghr/chromosomes</ref> There is also a telomere region within the human chromosome two, as well as a non-functional second centromere. <ref>http://www.pnas.org/content/88/20/9051.abstract</ref> This indicates a historical chromosome fusion event.
-==Number of Chromosomes==
-The chromosome number varies in different species. In humans there are 46 chromosomes, or 23 pairs of chromosomes ([[diploid]]), in every cell except the mature egg and sperm which have a set of 23 chromosomes ([[haploid]]).
-==Cell Division==
-Chromosomes are visible only during cell division, when the DNA is super coiled and condensed to facilitate distribution into daughter cells. Cell division in somatic cells (mitosis) results in the creation of daughter cells with the same number of chromosomes as the original cell, a total of 46 chromosomes in a human. Cell division in the germ cells, eggs and sperm (meiosis), results in the creation of daughter cells with half the number of chromosomes as the original cell (haploid cells). This reduction in the number of chromosomes is important so that the original number of chromosomes is restored following fertilization of the egg by the sperm.
-==Karyotype Analysis==
-The chromosome constitution of an individual, karyotype, can be analyzed following tissue culture of an appropriate sample. The most commonly used sample is blood (using the white blood cells or lymphocytes) since it is the most accessible. However, other samples are used depending upon the indication: amniotic fluid cells, to analyze the karyotype of the fetus; products of conception, to analyze the cause of a miscarriage or stillbirth; bone marrow cells, to diagnose the presence or type of leukemia; and skin, to determine the presence of another cell line (mosaicism).
-Cell division is arrested during metaphase, when the chromosome material is condensed. Following hypotonic treatment and fixation, the cells are dropped on a slide and then stained. At least 20 metaphase spreads are analyzed and 2 or 3 metaphase spreads are photographed. The chromosomes are arranged from largest to smallest to create a karyotype.
-Chromosomes vary in size and in shape. The pairs of [[Autosome|autosomal chromosomes]] are arranged in a karyotype from the biggest to the smallest. The [[sex chromosomes]] are placed to the right of the smallest autosomes. Chromosomes vary in shape depending upon the position of the [[centromere]], the structure that holds the two arms of the chromosomes together. If the [[centromere]] is in the middle, the chromosome is metacentric and the chromosome arms are equal in size. If the centromere is off center, the chromosome is submetacentric with a short arm labeled p (for petite) and a long arm labeled q (the next letter after p). If the centromere is close to the end, the chromosome is acrocentric and the very short arm consists of a stalk and a knob (satellite). Based upon size and shape, human chromosomes are divided into eight groups: A (1 to 3), B (4 and 5), C (6 to 12), D (13 to 15), E (16 to 18), F (19 and 20), G (21 and 22) and the sex chromosomes, XX in females and XY in males.
-Chromosomes are further identified by banding patterns created by specialized staining procedures to produce G bands, R bands, C bands, etc. The choice of staining procedure depends upon the information that is desired. Most commonly, chromosomes are stained with trypsin-Giemsa to produce the G-banded pattern. The banding pattern is determined by the degree of chromatin condensation and the specific DNA-protein present at the different sites on the chromosome. These banding patterns are distinct and consistent for each chromosome. Thus, one can reliably identify the chromosome pairs (e.g., although of the same size and shape, the six acrocentric chromosomes in the D group can be differentiated into pairs 13, 14 or 15 based upon banding patterns). The bands are individually numbered (e.g., 11q23 refers to band #23 on the long arm q of chromosome #11). :<ref>http://www.vivo.colostate.edu/hbooks/genetics/medgen/chromo/cytotech.html</ref>
-==References==
-{{reflist}}
-[[Category:Biology]]

Difference between revisions of "Chromosomes"

Latest revision as of 21:29, October 19, 2011

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