Changes

::::::::“Not-C” does not support “the paper says “Not-C”” and the circularity is broken.--[[User:Toffeeman|Toffeeman]] 09:07, 1 August 2008 (EDT)

::::::::“Not-C” does not support “the paper says “Not-C”” and the circularity is broken.--[[User:Toffeeman|Toffeeman]] 09:07, 1 August 2008 (EDT)

:::::::::In the third experiment, additional clones were used. They write: "Clones were chosen to maximize the diversity of colony morphologies from each sample, in case only certain types could produce Cit+ mutants." This is different from the selection of clones in the first and second experiments. Also in contrast to the second experiment which grew cells in DM500 media, they used DM1000 and DM2000 media to grow the cells in the third replay. Cells were plated at higher densities in the third replay experiment.

:::::::::In the third experiment, additional clones were used. The authors write: "Clones were chosen to maximize the diversity of colony morphologies from each sample, in case only certain types could produce Cit+ mutants." This is different from the selection of clones in the first and second experiments. Also in contrast to the second experiment which grew cells in DM500 media, they used DM1000 and DM2000 media to grow the cells in the third replay. Cells were plated at higher densities in the third replay experiment.

:::::::::As the authors comment, when they designed the third replay conditions, they assumed that their changes would not cause significant differences. Still, there clearly were differences in the results and there were some differences in the conditions. Note that if, as Andy initially suggested, the initial cultures were contaminated with Cit+ cells, one might expect that contamination to scale as well between the second and third experiments.

:::::::::As the authors comment, when they designed the third replay conditions, they assumed that their changes would not cause significant differences. Still, there clearly were differences in the results and there were some differences in the conditions. Note that if, as Andy initially suggested, the initial cultures were contaminated with Cit+ cells, one might expect that contamination to scale as well between the second and third experiments.