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In a '''light microscope''' (LM), visible [[light]] is passed through the specimen and then through glass lenses. The lenses refract (bend) the light so that the image of the specimen is magnified. Light microscopes were first used by [[Renaissance]] scientists and are still widely used in biology.<ref>http://www.ruf.rice.edu/~bioslabs/methods/microscopy/microscopy.html</ref>   

In a '''light microscope''' (LM), visible [[light]] is passed through the specimen and then through glass lenses. The lenses refract (bend) the light so that the image of the specimen is magnified. Light microscopes were first used by [[Renaissance]] scientists and are still widely used in biology.<ref>http://www.ruf.rice.edu/~bioslabs/methods/microscopy/microscopy.html</ref>   

Some important parameters in microscopy are magnification and resolution. Magnification is the ratio of an object's image size to its real size. Resolution is a measure of the clarity of an object. Another important parameter is contrast, which accentuates differences in parts of the sample.<ref>Campbell, Neil A., and Jane B. Reece. Biology. San Francisco: Benjamin Cummings, 2002. Print.</ref>

Some important parameters in microscopy are magnification and resolution. Magnification is the ratio of an object's image size to its real size. Resolution is a measure of the clarity of an object. Another important parameter is contrast, which accentuates differences in parts of the sample.<ref>Campbell, Neil A., and Jane B. Reece. Biology. San Francisco: Benjamin Cummings, 2002.</ref>

The light microscope has a limited resolving power; it cannot resolve detail finer than .2 micrometers, or the size of a small [[bacterium]].  

The light microscope has a limited resolving power; it cannot resolve detail finer than .2 micrometers, or the size of a small [[bacterium]].  

==References==

==References==