Changes

The NewFlo phenotype differs from that of FLO1 in several ways. Firstly NewFlo flocculation is inhibited by mannose, glucose and maltose (Stratford and Assinder, 1991; Masy, 1992; Rhymes, 1999). Secondly the NewFlo lectin is putatively encoded by the FLO10 gene (Guo et al, 2000; Smart, 2001) and is not expressed until stationary phase onset (Stratford, 1989; Stratford and Assinder, 1991; D’Hautcourt and Smart, 1999). Thirdly lectin maturation occurs some fourteen hours after the cessation of cell division (Stratford, 1989; Stratford and Assinder, 1991; Masy 1992; D’Hautcourt and Smart, 1999) and is therefore not concomitant with entry into stationary phase, although this is strain dependent (D’Hautcourt and Smart, 1999; Verstrepen et al, 2003).  

The NewFlo phenotype differs from that of FLO1 in several ways. Firstly NewFlo flocculation is inhibited by mannose, glucose and maltose (Stratford and Assinder, 1991; Masy, 1992; Rhymes, 1999). Secondly the NewFlo lectin is putatively encoded by the FLO10 gene (Guo et al, 2000; Smart, 2001) and is not expressed until stationary phase onset (Stratford, 1989; Stratford and Assinder, 1991; D’Hautcourt and Smart, 1999). Thirdly lectin maturation occurs some fourteen hours after the cessation of cell division (Stratford, 1989; Stratford and Assinder, 1991; Masy 1992; D’Hautcourt and Smart, 1999) and is therefore not concomitant with entry into stationary phase, although this is strain dependent (D’Hautcourt and Smart, 1999; Verstrepen et al, 2003).  

The MI phenotype appears to occur in ale ([[Saccharomyces cerevisiae]], but not lager ([[saccharomyces pastorianus]]) strains (Masy et al, 1992; Dengis and Rouxhet, 1997; Jin and Speers, 1999) and is considered to be a rare phenotype. The FLO11 gene has however been identified as being essential for flocculation in S. bayanus (Ishigami et al, 2004) and characteristics such as invasive growth and pseudohyphal formation in Saccharomyces cerevisiae (Lo and Dranginis, 1998; Gagiano et al, 1999; Gancedo, 2001; Gagiano et al, 2002; Gagiano et al, 2003; Verduzco-Luque et al, 2003; Vivier et al, 2003; Guldener et al, 2004). Although this flocculation phenotype has not been fully characterised, it is differentiated from other flocculation phenotypes by a lack of inhibition of the lectin like reaction in the presence of mannose (Dengis and Rouxhet, 1997; Guo et al, 2000; Smart, 2001).

The MI phenotype appears to occur in ale ([[Saccharomyces cerevisiae]]), but not lager ([[saccharomyces pastorianus]]) strains (Masy et al, 1992; Dengis and Rouxhet, 1997; Jin and Speers, 1999) and is considered to be a rare phenotype. The FLO11 gene has however been identified as being essential for flocculation in S. bayanus (Ishigami et al, 2004) and characteristics such as invasive growth and pseudohyphal formation in Saccharomyces cerevisiae (Lo and Dranginis, 1998; Gagiano et al, 1999; Gancedo, 2001; Gagiano et al, 2002; Gagiano et al, 2003; Verduzco-Luque et al, 2003; Vivier et al, 2003; Guldener et al, 2004). Although this flocculation phenotype has not been fully characterised, it is differentiated from other flocculation phenotypes by a lack of inhibition of the lectin like reaction in the presence of mannose (Dengis and Rouxhet, 1997; Guo et al, 2000; Smart, 2001).

[[Category:Chemistry]]

[[Category:Chemistry]]