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Nucleotide excision repair (view source)
Revision as of 15:34, January 7, 2008
, 15:34, January 7, 2008New page: Nucleotide excision repair is a process that mends pyrimidine dimers. In this repair pathway, an oligonucleotide containing the lesion is excised from the DNA and the resultin...
Nucleotide excision repair is a process that mends [[pyrimidine]] dimers. In this repair pathway, an [[oligonucleotide]] containing the lesion is excised from the [[DNA]] and the resulting single-strand gap is filled in.
In E. coli, pyrimidine dimers are recognized by a multisubunit enzyme, the product of the uvrA, uvrB, and uvrC genes. This UvrABC endonuclease, is an [[ATP]]-dependent reaction, cleaves the dimer-containt DNA strand at the sevent and fourth phosphodiester bonds on the dimer's 5' and 3' sides, respectively. The excised oligonucleotide is replaced through the action of a DNA polymerase, most probably Pol I, followed by that of DNA ligase.
In E. coli, pyrimidine dimers are recognized by a multisubunit enzyme, the product of the uvrA, uvrB, and uvrC genes. This UvrABC endonuclease, is an [[ATP]]-dependent reaction, cleaves the dimer-containt DNA strand at the sevent and fourth phosphodiester bonds on the dimer's 5' and 3' sides, respectively. The excised oligonucleotide is replaced through the action of a DNA polymerase, most probably Pol I, followed by that of DNA ligase.