Changes

'''Nucleotide excision''' repair is a process that mends [[pyrimidine]] dimers.  In this repair pathway, an [[oligonucleotide]] containing the lesion is excised from the [[DNA]] and the resulting single-strand gap is filled in.

'''Nucleotide excision''' repair is a process that mends [[pyrimidine]] dimers.  In this repair pathway, an [[oligonucleotide]] containing the lesion is excised from the [[DNA]] and the resulting single-strand gap is filled in.

In E. coli, pyrimidine dimers are recognized by a multisubunit enzyme, the product of the uvrA, uvrB, and uvrC genes.  This UvrABC endonuclease, is an [[ATP]]-dependent reaction, cleaves the dimer-containt DNA strand at the sevent and fourth phosphodiester bonds on the dimer's 5' and 3' sides, respectively.  The excised oligonucleotide is replaced through the action of a DNA polymerase, most probably Pol I, followed by that of DNA ligase.

In E. coli, pyrimidine dimers are recognized by a multisubunit enzyme, the product of the uvrA, uvrB, and uvrC genes.  This UvrABC endonuclease, is an [[ATP]]-dependent reaction, cleaves the dimer-contain DNA strand at the seventh and fourth phosphodiester bonds on the dimer's 5' and 3' sides, respectively.  The excised oligonucleotide is replaced through the action of a DNA polymerase, most probably Pol I, followed by that of DNA ligase.

[[Category:Biochemistry]]

[[Category:Biochemistry]]