Changes

Jump to navigation Jump to search

Conservapedia talk:Lenski dialog (view source)

Revision as of 13:15, June 27, 2008

2,389 bytes added ,  13:15, June 27, 2008
→‎Reply to comments in archive: examples of data that should be released
Line 32: Line 32:     
:::: I hope this has been somewhat instructive for you in explaining why a request for "further data" will not bear fruit beyond the small amount of data that Prof. Lenski says he will make available.  And why it is not reasonable to expect further data.  I am more than happy to respond to questions or requests for clarification if you would like.  -- [[User:Princetonian|Princetonian]] 23:50, 26 June 2008 (EDT)
 
:::: I hope this has been somewhat instructive for you in explaining why a request for "further data" will not bear fruit beyond the small amount of data that Prof. Lenski says he will make available.  And why it is not reasonable to expect further data.  I am more than happy to respond to questions or requests for clarification if you would like.  -- [[User:Princetonian|Princetonian]] 23:50, 26 June 2008 (EDT)
 +
 +
::::: For starters, most of the opposition to releasing the data has been the false claim that the data are too voluminous to release.  You are making the opposite claim, one which is more consistent with Lenski's paper: there is very little important data beyond what is in the 8-page paper.  But that doesn't withstand scrutiny either.
 +
 +
::::: The "meat" of the paper is this, and at a minimum the data should be released for it (pp. 2-3 from paper):
 +
 +
:::::: Evolution of Cit Function in Population Ara-3. The LTEE populations  are transferred daily into fresh medium, and the turbidity of each is checked visually at that time. [DATA ON THESE OBSERVATIONS?] Owing to the low concentration of glucose in DM25 medium [DATA?], the cultures are only slightly turbid when transferred. Occasional contaminants that grow on citrate have been seen over the 20 years of this experiment. [DATA?]  These contaminated cultures reach much higher turbidity owing to the high concentration of citrate in the medium, which allows the contaminants to reach high density.  (When contamination occurs, the affected population is restarted from the latest frozen sample.) [DATA FOR WHEN THAT OCCURRED?] After 33,127 generations, one population, designated Ara-3, displayed significantly elevated turbidity that continued to rise for several days (Fig. 1).[HIGHER RESOLUTION DATA UNDERLYING FIGURE?] A number [DATA?] of Cit clones were isolated from the population and checked for phenotypic markers characteristic of the ancestral E. coli strain used to start the LTEE: all [DATA?] were Ara, T5-sensitive,  and T6-resistant, as expected (2) [DATA ABOUT THESE AND OTHER CHARACTERISTICS?]. DNA seqencing also showed [DATA?] that Cit clones have the same mutations in the pykF and nadR genes as do clones from earlier generations of the Ara-3 population, and each of these mutations distinguishes this population from all [DATA?] of the others (30). Therefore, the Cit variant arose within the LTEE and is not a contaminant [THIS CONCLUSION NEEDS TO BE CHECKED BY INDEPENDENT REVIEW OF THE ACTUAL DATA, WHICH I DOUBT OCCURRED IN THE BRIEF PEER REVIEW OF THIS PAPER]
 +
 +
::::: Note, by the way, how the opponents of data disclosure seem to have no idea about what data Lenski actually has, which raises further questions about the merits of the conclusion.--[[User:Aschlafly|Aschlafly]] 09:15, 27 June 2008 (EDT)
    
----
 
----
Aschlafly
Anthony, Irenaeus, Siteadmin, Bureaucrats, Check users, nsAm_Govt_101RO, nsAm_Govt_101RW, nsAm_Govt_101_ta, nsJudgesRO, nsJudgesRW, nsJudges_talkRO, nsJudges_talkRW, nsTeam2RO, nsTeam2RW, nsTeam2_talkRO, nsTeam2_talkRW, oversight, Administrators
125,912

edits

Retrieved from "https://www.conservapedia.com/Special:MobileDiff/482731"

Navigation menu