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Line 39: − DATA FOR WHEN THAT OCCURRED, AND HOW OFTEN?+ − IN FACT, TWO REQUESTS WERE MADE DIRECTLY TO LENSKI FOR DATA, WITHOUT SUCCESS. HIS [[Conservapedia:Lenski_dialog#Second_reply|SECOND RESPONSE]] WAS RUDE AND INSULTING.+ Line 51:
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Line 75: − DATA ABOUT THESE AND OTHER CHARACTERISTICS?+ − WHAT IS MISSING FROM DISCLOSURE ARE THE DATA THAT THE MARKERS WERE ACTUALLY OBSERVED IN A RELIABLE AND CONCLUSIVE MANNER IN THIS STUDY.+ Line 91:
Line 91: − NO ONE ASKED FOR THE DATA TO BE PRINTED IN THE PAPER. WHAT IS MISSING IS DISCLOSURE OF THE DATA ON THE WEBSITE, OR UPON PUBLIC REQUEST.+ Line 99:
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Line 113: − DATA DISTINGUISHING "THIS POPULATION FROM ALL THE OTHERS"?+ − Line 122:
Line 121: − THE ASTOUNDINGLY SHORT 14-DAY [[peer review | PEER REVIEW]] PERIOD, MEASURED FROM THE DAY THE PAPER WAS SENT OUT FOR REVIEW TO THE DAY OF FORMAL CONTRIBUTION BY LENSKI AFTER ACCEPTANCE, RAISES QUESTIONS AS TO WHETHER THERE WAS ANY MEANINGFUL PEER REVIEW OF THIS AT ALL.+
Don't always have to write in all caps. This is much more civil.
'''Richard Lenski''' is a professor of microbial [[ecology]] at Michigan State [[University]]. He holds a [[doctorate]] from the [[University of North Carolina]] and a B.A. from Oberlin College but does not disclose his fields for those degrees on his own biographical sketch.
'''Richard Lenski''' is a professor of microbial [[ecology]] at Michigan State [[University]]. He holds a [[doctorate]] from the [[University of North Carolina]] and a B.A. from Oberlin College but does not disclose his fields for those degrees on his own biographical sketch.<ref>https://www.msu.edu/~lenski/</ref>
Lenski is best known for his claim to have observed [[evolution]] of [[E. coli]] in a long-term laboratory study, insisting that it was not due to contamination. His 2008 paper asserting his claims was [[peer review]]ed in a mere 14 days, sparking questions from the [[user:Aschlafly|owner]] of this site<ref>See [[Conservapedia talk:Lenski dialog]].</ref> about the thoroughness of the review.
Lenski is best known for his claim to have observed [[evolution]] of [[E. coli]] in a long-term laboratory study, insisting that it was not due to contamination. His 2008 paper asserting his claims was [[peer review]]ed in a mere 14 days, sparking questions from the [[user:Aschlafly|owner]] of this site<ref>See [[Conservapedia talk:Lenski dialog]].</ref> about the thoroughness of the review.
Evolution of Cit Function in Population Ara-3. The LTEE populations are transferred daily into fresh medium, and the turbidity of each is checked visually at that time. ...
Evolution of Cit Function in Population Ara-3. The LTEE populations are transferred daily into fresh medium, and the turbidity of each is checked visually at that time. ...
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Data on these observations?
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...the cultures are only slightly turbid when transferred. Occasional contaminants that grow on citrate have been seen over the 20 years of this experiment.
...the cultures are only slightly turbid when transferred. Occasional contaminants that grow on citrate have been seen over the 20 years of this experiment.
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Data? When and how many?
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These contaminated cultures reach much higher turbidity owing to the high concentration of citrate in the medium, which allows the contaminants to reach high density. (When contamination occurs, the affected population is restarted from the latest frozen sample.)
These contaminated cultures reach much higher turbidity owing to the high concentration of citrate in the medium, which allows the contaminants to reach high density. (When contamination occurs, the affected population is restarted from the latest frozen sample.)
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Data for when that occurred, and how often?
A Lenski defender says, "If interested in a thorough review, contact the group directly, with a legitimate request for data. Otherwise, the disclaimer is a more than adequate description of the sample handling process."
A Lenski defender says, "If interested in a thorough review, contact the group directly, with a legitimate request for data. Otherwise, the disclaimer is a more than adequate description of the sample handling process."
In fact, two requests were made directly to Lenski for data, without success. His second response was rude and insulting.
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After 33,127 generations, one population, designated Ara-3, displayed significantly elevated turbidity that continued to rise for several days (Fig. 1).
After 33,127 generations, one population, designated Ara-3, displayed significantly elevated turbidity that continued to rise for several days (Fig. 1).
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Higher resolution data underlying figure not provided despite request.
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A number...
A number...
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Data? How many? Statistically significant?
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of Cit clones were isolated from the population and checked for phenotypic markers characteristic of the ancestral E. coli strain used to start the LTEE: all
of Cit clones were isolated from the population and checked for phenotypic markers characteristic of the ancestral E. coli strain used to start the LTEE: all
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Data? How many? Statistically significant?
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...were Ara, T5-sensitive, and T6-resistant, as expected (2)
...were Ara, T5-sensitive, and T6-resistant, as expected (2)
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Data about these and other characteristics?
A Lenski defender says, "Interested parties should consult the literature on these topics. It is not within the scope of this paper to address the significance of these markers."
A Lenski defender says, "Interested parties should consult the literature on these topics. It is not within the scope of this paper to address the significance of these markers."
What is missing from disclosure are the data that the markers were actually observed in a reliable and conclusive manner in this study.
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A Lenski defender says, "The inclusion of this data would be considerably more extensive than appropriate for the inclusion in this paper."
A Lenski defender says, "The inclusion of this data would be considerably more extensive than appropriate for the inclusion in this paper."
No one asked for the data to be printed in the paper. What is missing is disclosure of the data on the website, or upon public request.
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...that Cit clones have the same mutations in the pykF and nadR genes as do clones from earlier generations of the Ara-3 population, ...
...that Cit clones have the same mutations in the pykF and nadR genes as do clones from earlier generations of the Ara-3 population, ...
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Data about these and other characteristics?
A Lenski defender cites to general information unrelated to this study: Available in other publications,
A Lenski defender cites to general information unrelated to this study: Available in other publications,
...and each of these mutations distinguishes this population from all the others (30).
...and each of these mutations distinguishes this population from all the others (30).
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Data distinguishing "This population from all the others"?
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Therefore, the Cit variant arose within the LTEE and is not a contaminant.
Therefore, the Cit variant arose within the LTEE and is not a contaminant.
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The astoundingly short 14-day [[peer review | PEER REVIEW]] period, measured from the day the paper was sent out for review to the day of formal contribution by Lenski after acceptance, raises questions as to whether there was any meaningful peer review of this at all.
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