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Talk:Flaws in Richard Lenski Study (view source)
Revision as of 00:12, July 15, 2008
, 00:12, July 15, 2008→Richard Lenski incorrectly included generations of the E. coli already known to contain Cit+ variants in his experiments.[3] Once these generations are removed from the analysis, the data disprove Len
::Oops, you're right, Argon. Cit+ wasn't dominant at generation 32,000, so replica plating might not be the best option. Still, in the case of almost 20% Cit+, I think replica plating might be labor reducing. I'm a biochemist by training, not a microbiologist, but I'm sure there are other methods that could be used. For example, Christensen's agar appears to provide a sensitive, colorimetric method of identifying even weakly citrate-utilizing colonies, so one might be able to plate cells on a Christensen's agar plate and pick uncolored colonies. Again though, I'm no microbiologist so I don't know the best method. However, there are ways to easily pick Cit- clones from later generations in this case. That said, I think that this specific "flaw" cited in Lenski's paper should be removed from the main article. Clearly, Cit+ were not used in the replays from later generations, so Conservapedia's objection is totally baseless.[[User:Gerlach|Gerlach]] 19:19, 14 July 2008 (EDT)
::Oops, you're right, Argon. Cit+ wasn't dominant at generation 32,000, so replica plating might not be the best option. Still, in the case of almost 20% Cit+, I think replica plating might be labor reducing. I'm a biochemist by training, not a microbiologist, but I'm sure there are other methods that could be used. For example, Christensen's agar appears to provide a sensitive, colorimetric method of identifying even weakly citrate-utilizing colonies, so one might be able to plate cells on a Christensen's agar plate and pick uncolored colonies. Again though, I'm no microbiologist so I don't know the best method. However, there are ways to easily pick Cit- clones from later generations in this case. That said, I think that this specific "flaw" cited in Lenski's paper should be removed from the main article. Clearly, Cit+ were not used in the replays from later generations, so Conservapedia's objection is totally baseless.[[User:Gerlach|Gerlach]] 19:19, 14 July 2008 (EDT)
::All appropriate descriptions. For generating estimate of the population distribution (Cit-/Cit+) in a culture, replica plating would certainly be applicable. The main point is that identifying and isolating Cit- clones from the generations used in the experiment is straightforward. I agree that it would be a mistake to keep that objection in the article (actually that thread runs across several points in the article).--[[User:Argon|Argon]] 20:12, 14 July 2008 (EDT)
== 1. Lenski's "historical contingency" hypothesis, as specifically depicted in Figure 3, is contradicted by the data presented... ==
== 1. Lenski's "historical contingency" hypothesis, as specifically depicted in Figure 3, is contradicted by the data presented... ==