Changes

:::: 2. The hypotheses suggest a fixed mutation rate, but the failure of the mutations to increase based on scale disproves the hypotheses.  If the Second and Third experiments were so dissimilar to the First Experiment that they cannot be compared for scale, then it was error to treat them as similar for the statistical analysis.

:::: 2. The hypotheses suggest a fixed mutation rate, but the failure of the mutations to increase based on scale disproves the hypotheses.  If the Second and Third experiments were so dissimilar to the First Experiment that they cannot be compared for scale, then it was error to treat them as similar for the statistical analysis.

:::: 3. The paper incorrectly applied a Monte Carlo resampling test to exclude the null hypothesis for rarely occurring events.  The Third Experiment results are entirely consistent with the null hypothesis.  

:::: 3. The paper incorrectly applied a Monte Carlo resampling test to exclude the null hypothesis for rarely occurring events.  The Third Experiment results are entirely consistent with the null hypothesis.  

:::: 4. It was error to include generations of the E. coli already known to contain trace Cit+ variants in his experiments, and the otherwise improbable occurrence of four Cit+ variants from the 32,000 generation in the Second Experiment strongly suggests an origin from undetected pre-existing Cit+ variants.

:::: 4. It was error to include generations of the E. coli already known to contain trace Cit+ variants, and the otherwise highly improbable occurrence of four Cit+ variants from the 32,000 generation in the Second Experiment suggests an origin from undetected pre-existing Cit+ variants.

:::: 5. The Third Experiment was erroneously combined with the other two based on outcome rather than sample size, thereby yielding a false claim of overall statistical significance.

:::: 5. The Third Experiment was erroneously combined with the other two experiments based on outcome rather than sample size, thereby yielding a false claim of overall statistical significance.

::: The underlying data for this taxpayer-funded has not been released to the public, despite requests to do so.

::: The underlying data for this taxpayer-funded has not been released to the public, despite requests to do so.