Changes

Evolution of Cit Function in Population Ara-3. The LTEE populations are transferred daily into fresh medium, and the turbidity of each is checked visually at that time. ...

Evolution of Cit Function in Population Ara-3. The LTEE populations are transferred daily into fresh medium, and the turbidity of each is checked visually at that time. ...

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Data on these observations?

Why does conservapedia fail so much?

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...the cultures are only slightly turbid when transferred. Occasional contaminants that grow on citrate have been seen over the 20 years of this experiment.

...the cultures are only slightly turbid when transferred. Occasional contaminants that grow on citrate have been seen over the 20 years of this experiment.

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Data? When and how many?

Why does conservapedia fail so much?

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These contaminated cultures reach much higher turbidity owing to the high concentration of [[citrate]] in the medium, which allows the contaminants to reach high density. (When contamination occurs, the affected population is restarted from the latest frozen sample.)  

These contaminated cultures reach much higher turbidity owing to the high concentration of [[citrate]] in the medium, which allows the contaminants to reach high density. (When contamination occurs, the affected population is restarted from the latest frozen sample.)  

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Data for when that occurred, and how often?

Why does conservapedia fail so much?

A Richard Lenski defender says, "If interested in a thorough review, contact the group directly, with a legitimate request for data.  Otherwise, the disclaimer is a more than adequate description of the sample handling process."

A Richard Lenski defender says, "If interested in a thorough review, contact the group directly, with a legitimate request for data.  Otherwise, the disclaimer is a more than adequate description of the sample handling process."

In fact, two requests were made directly to Lenski for data, without success. His [[Conservapedia:Lenski dialog#Second_Reply|second response]] was rude and insulting.

In fact, two requests were made directly to Lenski for data, without success. His [[Conservapedia:Lenski dialog#Second_Reply|second response]] was rude and insulting (Why does conservapedia fail so much?).

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After 33,127 generations, one population, designated Ara-3, displayed significantly elevated turbidity that continued to rise for several days (Fig. 1).

After 33,127 generations, one population, designated Ara-3, displayed significantly elevated turbidity that continued to rise for several days (Fig. 1).

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A number...

A number...

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Data? How many? Statistically significant?

Why does conservapedia fail so much?

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of Cit clones were isolated from the population and checked for phenotypic markers characteristic of the ancestral E. coli strain used to start the LTEE: all

of Cit clones were isolated from the population and checked for phenotypic markers characteristic of the ancestral E. coli strain used to start the LTEE: all

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Data? How many? Statistically significant?

Why does conservapedia fail so much?

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...were Ara, T5-sensitive, and T6-resistant, as expected (2)

...were Ara, T5-sensitive, and T6-resistant, as expected (2)

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Data about these and other characteristics?

Why does conservapedia fail so much?

A Richard Lenski defender says, "Interested parties should consult the literature on these topics.  It is not within the scope of this paper to address the significance of these markers."

A Richard Lenski defender says, "Interested parties should consult the literature on these topics.  It is not within the scope of this paper to address the significance of these markers."

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DNA sequencing also showed...

DNA sequencing also showed...

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DATA??

Why does conservapedia fail so much?

A Richard Lenski defender says, "The inclusion of this data would be considerably more extensive than appropriate for the inclusion in this paper."

A Richard Lenski defender says, "The inclusion of this data would be considerably more extensive than appropriate for the inclusion in this paper."

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...that Cit clones have the same mutations in the pykF and nadR genes as do clones from earlier generations of the Ara-3 population, ...  

...that Cit clones have the same mutations in the pykF and nadR genes as do clones from earlier generations of the Ara-3 population, ...  

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Data about these and other characteristics?

Why does conservapedia fail so much?

A Lenski defender cites to general information unrelated to this study: Available in other publications,

A Lenski defender cites to general information unrelated to this study: Available in other publications,

A Richard Lenski supporter also cites an earlier claim (perhaps also unsupported by public release of the data) by Lenski's group: See also the reference for the sequence identification which Blount ''et al.'' list as reference #30 in their paper: Woods, R., D. Schneider, C. L. Winkworth, M. A. Riley, and R. E. Lenski. 2006. Tests of parallel molecular evolution in a long-term experiment with Escherichia coli. PNAS, USA 103:9107-9112.<ref>http://www.pnas.org/cgi/content/abstract/0602917103v1</ref>.

A Richard Lenski supporter also cites an earlier claim (perhaps also unsupported by public release of the data) by Lenski's group: See also the reference for the sequence identification which Blount ''et al.'' list as reference #30 in their paper: Woods, R., D. Schneider, C. L. Winkworth, M. A. Riley, and R. E. Lenski. 2006. Tests of parallel molecular evolution in a long-term experiment with Escherichia coli. PNAS, USA 103:9107-9112.<ref>http://www.pnas.org/cgi/content/abstract/0602917103v1</ref>.

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...and each of these mutations distinguishes this population from all the others (30).  

...and each of these mutations distinguishes this population from all the others (30).  

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Data distinguishing "This population from all the others"?

Why does conservapedia fail so much?

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Therefore, the Cit variant arose within the LTEE and is not a contaminant.

Therefore, the Cit variant arose within the LTEE and is not a contaminant.

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Cit⁺ cells constituted [about] 0.5% of the population at generation 31,500, then 15% and 19% in the next two samples, but only [about] 1.1% at generation 33,000.

Cit⁺ cells constituted [about] 0.5% of the population at generation 31,500, then 15% and 19% in the next two samples, but only [about] 1.1% at generation 33,000.

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What were the sample sizes and where are the actual data for this sampling?

Why does conservapedia fail so much?

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