Drug resistance is also used in many molecular biology techniques. A plasmid containing a gene of interest and a gene for antibiotic resistance is transformed into a strain of bacteria (usually E.Coli) via chemical or electrical means. The bacteria is then plated onto media containing the antibiotic corresponding to the gene of antibiotic resistance. Only bacteria that have taken up the plasmid containing the antibiotic resistance gene can grow on the media. The plasmid containing the gene of interest can then be purified via alkaline lysis. | Drug resistance is also used in many molecular biology techniques. A plasmid containing a gene of interest and a gene for antibiotic resistance is transformed into a strain of bacteria (usually E.Coli) via chemical or electrical means. The bacteria is then plated onto media containing the antibiotic corresponding to the gene of antibiotic resistance. Only bacteria that have taken up the plasmid containing the antibiotic resistance gene can grow on the media. The plasmid containing the gene of interest can then be purified via alkaline lysis. |