| Washington Nationals
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| Infield
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| Catchers: Jesus Flores, Wil Nieves
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First basemen: Nick Johnson, Josh Willingham, Adam Dunn, Kory Casto
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| Second basemen: Anderson Hernandez, Ronnie Belliard, Willie Harris, Pete Orr
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Third basemen: Ryan Zimmerman, Alberto Gonzalez, Kory Casto
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| Shortstops: Cristian Guzman, Alberto Gonzalez, Anderson Hernandez
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| Outfield
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| Left fielders: Adam Dunn, Josh Willingham, Wily Mo Peña, Willie Harris
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Right fielders: Elijah Dukes, Austin Kearns
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| Center fielders: Lastings Milledge, Elijah Dukes, Willie Harris
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| Pitchers
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| Starting: John Lannan, Scott Olsen, Daniel Cabrera, Shairon Martis, Jordan Zimmermann
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| Relieving: Joel Hanrahan, Joe Beimel, Saul Rivera, Jason Bergmann, Steven Shell, Garrett Mock, Julian Tavarez
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| Other
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| Coaches:
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Manager(s): Manny Acta
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