Changes

:::: - Lenski's Third Experiment failed to support his hypothesis with statistical significance, even with the incorrect inclusion of the Cit+ variant generations.  The paper incorrectly combined the Third Experiment with the other two based on outcome rather than sample size, thereby yielding a false claim of overall statistical significance.

:::: - Lenski's Third Experiment failed to support his hypothesis with statistical significance, even with the incorrect inclusion of the Cit+ variant generations.  The paper incorrectly combined the Third Experiment with the other two based on outcome rather than sample size, thereby yielding a false claim of overall statistical significance.

:::: - The lag time between the potentiating mutation in the largest experiment disproves Lenski's implicit assumption that it likely occurred in proximity with the occurrence of the Cit+ variant.

:::: - The lag time between the potentiating mutation in the largest experiment disproves Lenski's implicit assumption that it likely occurred in proximity with the occurrence of the Cit+ variant.

:::: - Lenski has not disclosed key portions of his data, such as how thoroughly the samples were screened for pre-existing Cit⁺ variants.

:::: - Lenski has not disclosed key portions of his data, such as how thoroughly the samples were screened for pre-existing Cit⁺ variants at what specific generations these variants were actually observed in the replay experiments.  In fact, while the paper states the generation periods for the First (replay) Experiment, it does not disclose the generation periods for the Second and Third Experiments.

::: I am requesting that the authors of the paper respond to each of the flaws in this forum and explain the withholding of the data, and submit a revision or retraction of their paper if any are held valid.

::: I am requesting that the authors of the paper respond to each of the flaws in this forum and explain the withholding of the data, and submit a revision or retraction of their paper if any are held valid.